Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction.
نویسندگان
چکیده
Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels-Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.
منابع مشابه
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عنوان ژورنال:
- Chemical communications
دوره 50 74 شماره
صفحات -
تاریخ انتشار 2014